Elisa assignment

Lyme disease is the most common vector-borne disease in the United States. Lyme disease is caused by the bacterium Borrelia burgdorferi and rarely, Borrelia mayonii. It is transmitted to humans through the bite of infected blacklegged ticks. Typical symptoms include fever, headache, fatigue, and a characteristic skin rash called erythema migrans. 3 patients who are suspected of having lyme disease are tested using ELISA. All 3 patients show all the symptoms associated with lyme disease. The ELISA is carried out to check for the presence of antibodies against B. burgdorferi bacteria in the patient’s blood. To carry out the test, blood is drawn from each patient

  • What component of the blood contains antibodies against burgdorferi? (1 point)
  • The ELISA test kit used contains the following components. Plastic plate, wash buffer, + and – controls, burgdorferi flagella protein and color development reagent. Briefly LIST out the steps needed to carry out the ELISA (4 points)
  • The results obtained are shown below. The numbers represent absorbance values.
  1. Based on the results what can you conclude about the concentration of anti burgdorferi antibody in patient C vs B? (3 points)
  2. Can you arrive at this conclusion without the absorbance values? (1 point)
  3. Why is the serial dilution of patient samples important to your conclusion in part a)? (1 point)
  4. Provide a hypothesis regarding patient A results. Why is patient A’s results so different from C and B even though all 3 patients show symptoms of lyme disease. (HINT: The results are real and not due to experimental error. Read the description of the question above for clues) (EXTRA CREDIT +5 points)

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